Ratio Spectra First Order Derivative Method has been developed for simultaneous estimation of Diclofenac Sodium and Tolperisone Hydrochloride. The analytical signals were measured at maxima 244.0 nm and minima 232.80 nm for Diclofenac Sodium and Tolperisone Hydrochloride respectively in the first order derivative spectra (∆ λ = 8 nm and Scaling factor = 1) of the ratio spectra. The ratio spectra obtained by dividing the mixture spectra using 16 µg/ml TOL and 17 µg/ml DFS spectra as divisor for Diclofenac Sodium and Tolperisone Hydrochloride respectively. Beer’s law obeyed in concentration range of 3-31 µg/ml and 4-28 µg/ml in distilled water for Diclofenac Sodium and Tolperisone Hydrochloride respectively. The percent assay of marketed formulation was 99.58±1.148 % and 99.86±1.369 % of Diclofenac Sodium and Tolperisone Hydrochloride respectively. The proposed methods are specific, selective, sensitive, accurate and precise since they are recommended for routine analysis.
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